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Human IgE/IgG4 Double-Color FluoroSpot

This optimized FluoroSpot assay reliably detects and measures human IgE and IgG4 secretions from either Antibody Secreting Cells (ASC) that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibodies, in frequencies as low as one in a million.

This assay utilizes the Capture and Detection Kits listed below.

Available in 2, 5, and 10 plate sets.

Components

Catalog #

Per Plate $

Ig Capture Kit

hB2F

$35.00

IgE Detection Kit

hB19

$90.00

IgG4 Detection Kit

hB44

$90.00

Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate. Please contact us before placing your order to ensure you have the proper configuration.

A flat rate for shipping will be applied to all orders. To use your company's FedEx account number, please contact us before placing your order.

Pricing is for US and Canada only and does not include shipping. All others, contact the distributor in your region.

Number of Plates

  • $430.00
 
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Human IgE/IgG4 Double-Color FluoroSpotHuman IgE/IgG4 Double-Color FluoroSpot

Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection Kit
Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Volume discounts available, please inquire.

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