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Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot

The ImmunoSpot IFN-γ/TNF-α/IL-2 assay is the first ever triple color mouse FluoroSpot

Detect cells secreting each cytokine individually or in combination(s) with accuracy comparable to single-color (single cytokine) analysis

Stimulated effector cells can be detected in frequencies as low as one in a million, allowing the enumeration of rare polyfunctional cells.

Analysis of the ImmunoSpot triple color FluoroSpot requires a fluorescence capable analyzer with the appropriate filters and light sources. CTL recommends any of the following Series 6 analyzers: FluoroCore, Ultra, Universal, Ultimate. (ImmunoSpot v7.0 or higher software is recommended for accurate analysis).

This assay utilizes the Capture and Detection Kits listed below.
Available in 2, 5, and 10 plate sets.


Catalog #

Per Plate $

IFN-γ/TNF-α/IL-2 Capture Kit



IFN-γ Detection Kit



TNF-α Detection Kit



IL-2 Detection Kit



A flat rate for shipping will be applied to all orders. To use your company's FedEx account number, please contact us before placing your order.

Pricing is for US and Canada only and does not include shipping. All others, contact the distributor in your region.

Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot
Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot Precoated

Number of Plates

  • $810.00
Click to view details
Mouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpotMouse IFN-γ/TNF-α /IL-2 Triple-Color FluoroSpot


Capture Kit
Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.

Detection Kit
Contains Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugate binds to the secondary antibody, forming a fluorescent spot when excited at the appropriate wavelength, using emission filters specific for detecting each of the wavelengths for the individual cytokines, with no spectral overlap between channels.

Volume discounts available, please inquire.

Cellular Technology Limited (CTL)
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