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Yes. The hardware and software of the ImmunoSpot® analyzers all support single- and double-color analysis. The optics and cameras of the S5 Micro Analyzers, however, are specifically enhanced for highest color fidelity and resolution.
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Image resolution varies depending on the Analyzer model. The S5 Core and S5 Macro allow for a resolution of 1600 x 1200. With CTL SmartSegmentation, the S5 Micro can provide resolution of 4800 x 4800 pixels per well. The S5 UV analyzer provides a resolution of 1388 x 1038 pixels per well.
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ImmunoSpot® Analyzers use a proprietary light configuration to evenly illuminate the entire well bottom surface. In addition, the S5 model line features an auto-light adjustment option which permit fine-tuning of the illumination for each well when the background varies strongly.
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With spots in all size categories, high resolution images provide more information on the spot morphology, thereby increasing the accuracy of the advanced spot morphology recognition features offered by ImmunoSpot® software (SmartSpot™). The spot size reflects the per cell productivity and secretion kinetics. Also, the strength of T cell stimulation defines the spot size; low avidity T cell recognition results in low productivity and small, faint spots. Granzyme B, TNF and IL-10 assays frequently produce small spots. In all these cases, using high-resolution image analysis can provide a definite advantage.
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The well images include the entire well bottom. Thanks to the auto-centering feature in the ImmunoSpot® analyzers, every single well is automatically centered using intelligent software algorithms. Thus, ImmunoSpot® analyzers compensate for manufacturing variations in the microtiter plates. Additionally, the ImmunoSpot® analyzers include Accurate Well Capture, a feature in which 100% of the well bottom surface is captured using autocentering with user definable safety margins.
Can all ImmunoSpot® analyzers be used for double-color analysis?