-
-
- First the cells need to be stimulated with an antigen is assay conditions (CTL recommends 300,000 cells per ml stimulated with peptides around 2µg/ml concentration.
- After 4 days cells need to be counted and viable cells be plated 3x105/ml and biological grade active IL-2 to be given at 2units/ml.
- Follow the cell lines growth for 5 days by looking at them with the microscope regularly
- Again count spin down and provide fresh IL-2 medium 3x105/ml
- Follow the cell lines growth for an additional 5 days and if they still look good and the cell number increases
- If the cell number start to decline cells need to be stimulated with antigen and irradiated autologus PBMC, this time without adding IL-2 into the medium and start from the beginning.
Literature:
Tary-Lehmann, M., Rolink, A.G., Lehmann, P.V., Nagy, Z.A., and Hurtenbach, U. Induction of graft versus host-associated immunodeficiency by CD4+ T-cell clones. J. Immunol. 1990. 145:2092-2098.
Rininsland F.H., Helms T., Asaad R.J., Boehm B.O. and Tary-Lehmann M. Granzyme B ELISPOT assay for ex vivo measurement of T cell immunity. J. Immunol. Methods 240:143-155, 2000.
-
Samples have been HLA-typed low and high resolution results are available for 3 Class I loci (A, B, C) and 4 Class II loci (DRB1, DQA, DQB and DP)
-
-
-
-
IFN-gamma
-
ACDA present in GAMBRO bags for blood collections.
-
COP Spectra machine
-
No, they are not.
-
No, we don’t have serological profiles.
-
Yes, we can deliver virtually any number of vials any time. We can also freeze the requested cells and deliver them within a customer-specified time range and release dates. Remember that CTL's proprietary freezing protocols ensure viability rates that are comparable to those of fresh PBMC samples.
-
CTL is using Dry Cryoshippers for shipping and ships them overnight with next morning delivery. These Cryoshippers are being used because the shipping cost is significantly lower then shipping them on Dry Ice and the shipping conditions are the same as storage conditions, therefore the character of the cells stays the same.
-
Frozen PBMC maintain >90% viability after thawing when CTL-Thawing Protocol is followed (see attached info: “Fresh vs. Frozen” poster)
-
For each donor in our PBMC database, the following tests have been performed:
ABO and RH Group/Type
HIV 1/2 Antibody
Irregular Antibody Screening
HTLV I/II Antibody
Hepatitis B Surface Antigen
HCV Antibody
Hepatitis B Core Antibody
Serologic Test for Syphilis
NAT HIV/HCV
What kind of Class I CEF-peptides are you testing for?