ImmunoSpot^® Kits and protocols were designed to minimize assay variables such as the substrate development time and serum. Serum is a major source for assay variability resulting from undefined suppressive or stimulatory effects. We offer ImmunoSpot^® Kits with a special serum-free medium, CTL-Test™, that we developed for standardized ELISPOT work. For those relying on cryopreserved PBMC, serum-free media are available for standardized freezing and thawing of PBMC. 

We also offer reference PBMC to verify that the assays are performing to expectations. Using this platform, the intra- and inter-laboratory variability can be reduced to a level that until recently had been considered “incredible” for a T cell assay (Zhang et al., J. Immunotoxicology, 2009, 6:227), and that can be readily verified today: for you, all it takes is to try the ImmunoSpot^® Kit!

Request your FREE trial kit today. 
We can also customize multi-color (up to 5-color) kits for you. Please inquire.

Kit Formats   |   Contact Us   |   Brochure   |   Order Form   |   Protocols

⇐ Back