04/03/2009 11:36

CTL Writes Book Chapter on Validating Reference Samples for Use in Cell-Based Assays

In a new book on cell-based assays, CTL discusses key considerations in validating an ELISPOT assay.

Cellular Technology Limited has contributed a key chapter to the book, Validation of Cell-Based Assays in the GLP Setting: A Practical Guide. This text is one of the latest offerings by Research and Markets, a leading source for international market research and market data.

This book describes the development, optimization and validation of various cell-based assays, such as cytokine ELISPOT and ELISA assays, for which validation procedures have been developed. In addition, it discusses the validation of these assays for select targets using step-by-step instructions. Chapter 9 ("Validating reference samples for comparison in a regulated ELISPOT assay") was written by CTL personnel Magdalena Tary-Lehmann, Christina D. Hamm, and Paul V. Lehmann. In this chapter, they discuss the utility and performance of the ELISPOT method, as well as key considerations in validating such assays.

Validation of Cell-based Assays in the GLP Setting is an invaluable reference source for pharmaceutical companies and biotechnology firms that need to evaluate drug targets derived from genomics and proteomics. It is also a useful reference for companies that use cell-based technologies for biomarker discovery and development.

In addition, the potential of biomarkers to facilitate the development of effective and safe drugs is being recognized as an integral part of all phases of drug development, and cell-based technologies are a critical part of biomarker discovery and development.

 

For more information visit www.researchandmarkets.com/reports/c89231.

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ELISPOT Readers


ImmunoSpot® Analyzers constitute CTL’s classic line of laboratory plate readers. Each Analyzer is designed for scanning and evaluating a wide range of microtiter plate-based bioassays—in particular, ELISPOT assays. ImmunoSpot® Analyzers are available in a variety of models, each one designed to make assay analysis faster, easier, and more efficient than ever before.

All CTL ImmunoSpot® Analyzers offer:

  • Uncompromised quality of analyzer components
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View CTL's Frequently Asked Questions on ELISPOT assays and ELISPOT instrumentation.

Learn why over 350 institutes worldwide have chosen CTL for their ELISPOT analysis needs.

 

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CTL-ImmunoSpot® S5 UV Analyzer

CTL's cutting-edge instrument for both visible light and ultraviolet-enabled work. The S5 UV Analyzer is CTL’s top-of-the-line model, being designed to support both visible light assays (e.g. ELISPOT, colony counting) and fluorescence-based FluoroSpot assays.  It is also well suited for cell viability tests, apotosis tests and in vivo/in vitro cytotoxicity measurements using cells labeled with fluorescent dyes.  Its mechanical design allows users to readily switch back and forth between the visible light and fluorescent modes, and its multi-filter design allows users to take advantage of the entire fluorescence-based spectrum.

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CTL-ImmunoSpot® S5 Micro Analyzer

CTL’s high-resolution model for microscopic analysis. The S5 Micro’s zoom lens permits seamless switching between 24-, 48-, and 96-well plate formats.  Its advanced optics allow users to zoom in further for more precise detail.  Even individual stained cells as small as 10 µm in diameter can be detected.

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CTL-ImmunoSpot® S5 Macro Analyzer

The versatile instrument for scanning the widest range of plate formats.  When it comes to scanning multiple plate formats, nothing beats the S5 Macro.  Its advanced optical design allows it to scan formats that range from 96-well plates to 100mm Petri dishes. When you need a versatile instrument that can analyze ELISPOT plates as well as many classical assays (bacterial, yeast, stem cell, and tumor colonies, as well as clonogenic and viral plaque assays), then the S5 Macro is the choice for you!

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