Reference Sample QC Sets™
CTL Reference Sample QC Sets™ are cryopreserved PBMC samples that have been characterized for IFN-γ production in response to certain viral antigens. Having high-quality reference samples is critical when performing standardized work, especially in a highly regulated environment.
The Reference Sample QC Sets™ have been specifically designed for use in assay validation and in protocol setup. They are also invaluable as internal controls for clinical trials, allowing researchers to use samples from donors with known reactivities to specific peptide antigens. Based on the validation results, the samples can be used to define cutoff values for acceptance of the assay. This makes CTL Reference Sample QC Sets™ a crucial component of assay standardization.
Many applications can benefit from the use of such PBMC reference samples. These include the following:
- ELISPOT assays
- Tetramer/Pentamer staining
- Intracytoplasmic cytokine staining (ICS)
- Cytokine ELISA assays
- Cytokine bead arrays (CBA)
- Cytokine protein arrays (CPA)
- Cytokine mRNA determinations (RT-PCR)
Each Reference Sample QC Set includes one vial of cyropreserved PBMC that bears a specified HLA allele. Also included are three vials of ready-to-use HLA Class I-restricted peptides: one that elicits a high-frequency response in the IFN-γ producing CD8 cells, one that produces a low frequency response, and one that elicits no response at all.
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