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ImmunoSpot® PUBLICATIONS BY THEME

For nearly 20 years, Prof. Lehmann and the team of scientists working with him have been focused on establishing the scientific foundations of ELISPOT assays and to test the utility of the technology in various fields of T cell diagnostics. CTL's peer-reviewed publications are listed here by theme, with the hopes of providing a useful reference for your research.

Publications Archive

Single-cell Resolution and Frequency

  • Hanson J,Sundararaman S, Caspell R, Karacsony E, Karulin AY, and Lehmann PV. ELISPOT assays in 384-well format: Up to 30 data points with one million cells. Cells. 2015; 4(1):71-83.
  • Karulin AY, Caspell R, Dittrich M, and Lehmann PV. Normal distribution of CD8+ T cell-derived ELISPOT counts within replicates justifies the reliance on parametric statistics for identifying positive responses. Cells. 2015; 4:96-111.
  • Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV. Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity. Cells. 2015; 7:4414-4437.
  • Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV. How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides? Viruses. 2012. 4:2636-2649.
  • Kuerten S, Batoulis H, Recks MS, Karacsony E, Zhang W, Subbramanian R, Lehmann, PV. Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays. Cells. 2012. 1:313-324.
  • Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S. ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators. J. Immunotoxicology. 2009. 6:227-34.
  • Ott PA, Tary-Lehmann M, Lehmann PV. The secretory IFN-γ response of single CD4 memory cells after activation on different antigen-presenting cell types. Clin. Immunol. 2007. 124:267-276.
  • Hofstetter HH, Karulin AY, Forsthuber TG, Ott PA, Tary-Lehmann M, Lehmann PV. The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice. J. Neuroimmunol. 2005. 170:105-14.
  • Hofstetter HH, Targoni OS, Karulin AY, Forsthuber TG, Tary-Lehmann M, Lehmann PV. Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis? J. Immunol. 2005. 174:4598.
  • Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M. A T cell clone’s avidity is a function of its activation state. J. Immunol. 2001. 167:1353.
  • Targoni OS, Baus J, Hofstetter HH, Hesse MD, Karulin AY, Boehm BO, Forsthuber TG, Lehmann PV. Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis. J. Immunol. 2001. 166:4757.
  • Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol. 2000. 164:3723.
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Measuring Th1, Th2, Th17, and Other Effector Lineages

  • Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV. Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity. Cells. 2015; 7:4414-4437.
  • Lehmann PV, Sundararaman S. When results of T cell immune monitoring match / do not match clinical outcomes of tumor vaccine trials - what more could and should we measure? In The Tumor ImmunoEnvironment, ed. M. Shurin, Victor Umansky & A.M. Malyguine, Springer 2013.
  • Karulin AY, Quast S, Hesse M, Lehmann PV. Neuroantigen-specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages. Cells. 2012. 1:576-596.
  • Anthony DD, Milkovich KA, Zhang W, Rodriguez B, Yonkers NL, Heeger PS, Tary-Lehmann M, Lehmann PV. Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT. Cells. 2012. 1:127-140.
  • Tigno JT, Lehmann PV, Tary-Lehmann M. Dissociated induction of cytotoxicity and DTH  by CFA and CpG. J. Immunother. 2009. 32:389.
  • Tigno-Aranjuez JT, Jaini R,  Tuohy VK, Lehmann PV, Tary-Lehmann M. Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cells. J. Immunol. 2009. 183(9): 5654-61.
  • Kuerten S, Asaad RJ, Schoenberger SP, Lehmann PV, Tary-Lehmann M. The TRAIL of helpless CD8+ T cells in HIV infection. AIDS Res Hum Retroviruses. 2008. 24: 1-9.
  • Hofstetter HH, Toyka KV, Tary-Lehmann M, Lehmann PV. Kinetics and Organ Distribution of IL-17-Producing CD4 Cells in Proteolipid Protein 139–151 Peptide-Induced Experimental Autoimmune Encephalomyelitis of SJL Mice.  J Immunol. 2007. 178: 1372-1378.
  • Hofstetter HH, Kovalovski A, Shive C, Lehmann PV, Forsthuber TG. Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood. J. Neuroimmunol. 2007. 187: 20-30.
  • Lichtenegger FS, Kuerten S, Faas S, Boehm BO, Tary-Lehmann M, Lehmann PV. Dissociation of EAE protective effect and allergic side reactions in tolerization with neuroantigen.  J. Immunol. 2007. 178:4749-56.
  • Kuerten S, Lichtenegger FS, Faas S, Angelov DN, Tary-Lehmann M, Lehmann PV. MBP-PLP fusion protein-induced EAE in C57BL/6 mice. J. Neuroimmunol. 2006. 177(1-2):99-111.
  • Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ. Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation. Transplantation. 2005. 80:1316.
  • Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV. Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin. Immunol. 2005. 115:102.
  • Eisenberg JC, Czinn SJ, Garhart CA, Redline RW, Bartholomae WC, Gottwein JM, Nedrud JG, Emancipator SE, Boehm BO, Lehmann PV, Blanchard TG. Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice. Infect. Immun. 2003. 71:1820.
  • Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J. Immunol. Methods. 2003. 279:111.
  • Karulin AY, Hesse MD, Yip HC, Lehmann PV. Indirect IL-4 pathway in type 1 immunity. 2002. J. Immunol. 168:545.
  • Radeke HH, Tschernig T, Karulin AY, Schumm G, Emancipator SN, Resch K, Tary-Lehmann M. CD4+ T cells recognizing specific antigen deposited in glomeruli cause glomerulonephritis-like kidney injury. Clin. Immunol. 2002. 104:161.
  • Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol.  2000. 164:3723.
  • Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. J. Immunol.  2000. 164:1862.
  • Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, Radeke H, Heeger PS, Trezza RP, Heinzel FP, Forsthuber T, Lehmann PV. Adjuvant-guided type-1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response. J. Immunol. 1999. 162:3942.
  • Tary-Lehmann M, Hricik DE, Justice AC, Potter NS, Heeger PS. Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure. Transplantation.  1998. 66:219.
  • Chu RS, Targoni OS, Krieg AM, Lehmann PV, Harding CV. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med.  1997. 186:1623.
  • Lehmann PV, D. Matesic, G. Benichou, and Heeger PS. Induction of T helper 2 immunity to an immunodominant allopeptide. Transplantation.  1997. 64:292.
  • Tian J, Lehmann PV, and D. L. Kaufman. Determinant spreading of T helper cell 2 (Th2) responses to pancreatic islet autoantigens. J. Exp. Med.  1997.186:2039.
  • Forsthuber T, Yip HC, Lehmann PV. Induction of TH1 and TH2 immunity in neonatal mice.Science. 1996. 271:1728.
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Cognate Cytokine Production by T Cells versus Bystander Cytokine Production

  • Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV. Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity. Cells. 2015; 7:4414-4437.
  • Anthony DD, Milkovich KA, Zhang W, Rodriguez B, Yonkers NL, Heeger PS, Tary-Lehmann M, Lehmann PV. Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT. Cells. 2012. 1:127-140.
  • Hofstetter HH, Karulin AY, Forsthuber TG, Ott PA, Tary-Lehmann M, Lehmann PV. The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice. J. Neuroimmunol. 2005. 170:105-14 .
  • Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J. Immunol. Methods. 2003. 279:111.
  • Karulin AY, Hesse MD, Yip HC, Lehmann PV. Indirect IL-4 pathway in type 1 immunity. J. Immunol. 2002. 168:545.

 

Measurements of Functional T Cell Avidity

  • Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV. How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides? Viruses. 2012. 4:2636-2649.
  • Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S. ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators. J. Immunotoxicology. 2009. 6:227-34. 
  • Hofstetter HH, Targoni OS, Karulin AY, Forsthuber TG, Tary-Lehmann M, Lehmann PV. Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis? J. Immunol. 2005. 174:4598.
  • Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M. A T cell clone’s avidity is a function of its activation state. J. Immunol. 2001. 167:1353.
  • Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, Radeke H, Heeger PS, Trezza RP, Heinzel FP, Forsthuber T, Lehmann PV. Adjuvant-guided type-1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response. J. Immunol. 1999. 162:3942.
  • Targoni OS Lehmann PV. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 1998. 187:2055.
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Cytokine Coexpression/Two-color ELISPOT Assays

  • Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV. Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity. Cells. 2015; 7:4414-4437.
  • Kuerten S, Wunsch M, Lehmann PV. Longitudinal T cell-derived IFN-γ/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis. J. Immunol. Methods. 2013. 398-399:68-75.
  • Karulin A, Quast S, Hesse M, Lehmann PV. Neuroantigen-specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages. Cells. 2012. 1:576-596.
  • Quast S, Zhang W, Shive C, Kovalovski D, Ott PA, Herzog BA, Boehm BO, Tary-Lehmann M, Karulin AY, Lehmann PV. IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays. Cell Immunol. 2005. 237:28.
  • Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV. Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin. Immunol. 2005. 115:102-14.
  • Karulin AY, Hesse MD, Yip HC, Lehmann PV. Indirect IL-4 pathway in type 1 immunity. J. Immunol. 2002. 168:545.
  • Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant. J. Immunol. 2000. 164:5771.
  • Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. J. Immunol. 2000. 164:1862.
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Spot Size Distribution

  • Sundararaman S, Karulin AY, Ansari T, BenHamouda N, Gottwein J, Laxmanan S, Levine SM, Loffredo JT, McArdle S, Neudoerfl C, Roen D, Silina K, Welch M, and Lehmann PV. High reproducibility of ELISPOT counts from nine different laboratories. Cells. 2015; 4(1):21-39.
  • Karulin AY, Karacsony K, Zhang W, Targoni OS, Moldovan I, Dittrich M, Sundararaman S, and Lehmann PV. ELISPOTs produced by CD8 and CD4 cells follow Log Normal size distribution permitting objective counting. Cells. 2015; 4:56-70.
  • Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV. Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity. Cells. 2015; 7:4414-4437.
  • Schlingmann TR, Shive CL, Targoni OS, Tary-Lehmann M, Lehmann PV. Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells. Cellular Immunology. 2009. 258:131-137.
  • Lehmann PV. Image analysis and data management of ELISPOT assay results. Methods Mol. Biol. 2005.302:117-32.
  • Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J. Immunol. Methods. 2003. 279:111.
  • Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M. A T cell clone’s avidity is a function of its activation state. J. Immunol. 2001. 167:1353.
  • Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. J. Immunol. 2000. 164:1862.
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Determinant Mapping/High-throughput Screening

  • Anthony DD, Lehmann PV. T-cell epitope mapping using the ELISPOT approach.Methods.  2003. 29:260.
  • Anthony DD, Valdez H, Post AB, Carlson NL, Heeger PS, Lehmann PV. Comprehensive determinant mapping of the hepatitis C-specific CD8 cell repertoire reveals unpredicted immune hierarchy.Clin. Immunol.  2002. 103:264.
  • Heeger PS, Valujskikh A, Lehmann PV. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen. J. Immunol.  2000. 165:1278.
  • Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, Radeke H, Heeger PS, Trezza RP, Heinzel FP, Forsthuber TG, Lehmann PV. Adjuvant-guided type-1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response. J. Immunol.  1999. 162:3942.
  • Targoni OS, Lehmann PV. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 1998. 187:2055.

 

Working with Peptides and Peptide Pools

  • Moldovan I, Targoni OS, Zhang W, Sundararaman S, Lehmann PV. How frequently are predicted peptides actually recognized by CD8 cells?Cancer Immunology, Immunotherapy. 2016. DOI 10.1007/s00262-016-1840-7.
  • Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV. How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?Viruses. 2012. 4:2636-2649.
  • Kuerten S, Pauly R, Rottlaender A, Rodi M, Gruppe TL, Addicks K, Tary-Lehmann M, Lehmann PV. Myelin-reactive antibodies mediate the pathology of MBP-PLP fusion protein MP4-induced EAE.Clin. Immunol., 2011. 140:54-62.
  • Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV. Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin. Immunol. 2005. 115:102.
  • Herzog BA, Ott PA, Dittrich MT, Quast S, Karulin AY, Kalbacher H, Karges W, Tary-Lehmann M, Lehmann PV, Boehm BO, Durinovic-Bello U. Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects. J. Autoimmun. 2004. 23:45.
  • Kleen TO, Asaad R, Landry, Boehm BO, Tary-Lehmann M. Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection. AIDS.  2004. 18:383.
  • Ott PA, Dittrich MT, Herzog BA, Guerkov R, Gottlieb PA, Putnam AL, Durinovic-Bello I, Boehm BO, Tary-Lehmann M, Lehmann PV. T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading. J. Clin. Immunol.  2004. 24:327.
  • Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, and Anthony DD. CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases. J. Immunol. Methods.  2004. 285:223.
  • Anthony DD, Lehmann PV. T-cell epitope mapping using the ELISPOT approach.Methods.  2003. 29:260.
  • Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods.  2003. 278:79.
  • Stern BV, Boehm BO, Tary-Lehmann M. Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity. J. Immunol. 2002. 168:6099.
  • Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M. A T cell clone’s avidity is a function of its activation state. J. Immunol. 2001. 167:1353.
  • Heeger PS, Valujskikh A, Lehmann PV. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen. J. Immunol. 2000. 165:1278.
  • Pelfrey CM, Rudick RA, Cotleur AC, Lee JC, Tary-Lehmann M, Lehmann PV. Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production. J. Immunol. 2000. 165:1641.
  • Targoni OS, Lehmann PV. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med.  1998. 187:2055.
  • Chu RS, Targoni OS, Krieg AM, Lehmann PV, Harding CV. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med. 1997. 186:1623.
  • Forsthuber T, Yip HC, Lehmann PV. Induction of TH1 and TH2 immunity in neonatal mice.Science.  1996. 271:1728. 
  • Lehmann PV, Cardinaux F, Appella E, Muller S, Falcioni F, Adorini L, Nagy ZA. Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking. Eur. J. Immunol.  1989. 19:1071.
  • Nagy ZA, Lehmann PV, Falcioni F, Muller S, Adorini L. Why peptides? Their possible role in the evolution of MHC-restricted T-cell recognition. Immunol. Today.  1989. 10:132.
  • Adorini L, Mulller S, Cardinaux F, Lehmann PV, Falcioni F, Nagy ZA. In vivo competition between self peptides and foreign antigens in T-cell activation. Nature. 1988. 334:623.
  • Adorini L, Sette A, Buus S, Grey HM, Darsley M, Lehmann PV, Doria G, Nagy ZA, Appella E. Interaction of an immunodominant epitope with Ia molecules in T-cell activation. Proc. Natl. Acad. Sci. U.S.A. 1988. 85:5181.
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Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC

  • Sundararaman S, Karulin AY, Ansari T, BenHamouda N, Gottwein J, Laxmanan S, Levine SM, Loffredo JT, McArdle S, Neudoerfl C, Roen D, Silina K, Welch M, and Lehmann PV. High reproducibility of ELISPOT counts from nine different laboratories. Cells. 2015; 4(1):21-39.
  • Kuerten S, Batoulis H, Recks MS, Karacsony E, Zhang W, Subbramanian R, Lehmann, PV. Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays. Cells.  2012. 1:313-324.
  • Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S. ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators. J. Immunotoxicology.  2009. 6:227-34.
  • Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D’Souza MP and the ELISPOT Collaborative Study Group. Results of an ELISPOT Proficiency Panel Conducted in 11 Laboratories Participating in International Human Immunodeficiency Virus Type 1 Vaccine trials. AIDS Research & Human Retroviruses. 2005. 21: 68-81.
  • Anthony DD, Lehmann PV. T-cell epitope mapping using the ELISPOT approach.Methods.  2003. 29:260.
  • Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods.  2003. 278:79.
  • Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS. Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool. Am. J. Transplant.  2002. 2:857
  • Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol.  2000. 164:3723.
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Fresh versus Cryopreserved PBMC

  • Wunsch M, Caspell R, Kuerten S, Lehmann PV, Sundararaman S. Serial measurements of apoptotic cell numbers provide better acceptance criterion for PBMC quality than a single measurement prior to the T cell assay. Cells. 2015. 4:40-55.
  • Kuerten S, Batoulis H, Recks MS, Karacsony E, Zhang W, Subbramanian R, Lehmann, PV. Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays. Cells. 2012. 1:313-324.
  • Ramachandran H, Laux J, Moldovan I, Caspell R, Lehmann PV, Subbramanian R. Optimal Thawing of Cryopreserved Peripheral Mononuclear Cells for Use in High-throughput Human Immune Monitoring Studies. Cells. 2012. 1:313-324.
  • Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods. 2003. 278:79.
  • Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS. Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool. Am. J. Transplant. 2002. 2:857.

 

Cytotoxicity, Granzyme B, and Perforin ELISPOT

  • Nowacki T, Bettenworth D, Ross M, Heidemann J, Lehmann PV, Lügering A. Cytomegalovirus (CMV)-specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease. Cells. 2012. 1:35-50.
  • Tigno JT,  Lehmann PV, Tary-Lehmann M. Dissociated induction of cytotoxicity and DTH  by CFA and CpG. J. Immunother. 2009. 32:389.
  • Nowacki TM, Kuerten S, Zhang W, Boehm BO, Lehmann PV, Tary-Lehmann M. Granzyme-B production, but not cytokine signatures distinguish recently activated CD8 memory cells from resting memory cells. Cell Immunol. 2007. 247:36-48. 
  • Kleen TO, Asaad R, Landry SJ, Boehm BO, Tary-Lehmann M. Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection. AIDS. 2004. 18:383.
  • Rininsland FH, Helms T, Asaad RJ, Boehm BO, Tary-Lehmann M. Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J. Immunol. Methods. 2000. 240:143.
  • Kuerten S, Kleen T, Assad RJ, Lehmann PV*, Tary-Lehmann M*. Dissociated production of perforin, granzyme B and IFN-γ by HIV-specific CD8+ cells in HIV infection. AIDS Research and Human Retroviruses. 2007. 24: 62-71. (*contributed equally)
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ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells

  • Kuerten S, Rottlaender A, Rodi M, Velasco VB Jr, Schroeter M, Kaiser C, Addicks K, Tary-Lehmann M, Lehmann PV. The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood. Clin. Immunol. 2010. 137(3):422-32.
  • Yonkers NL, Milkovich KA, Rodriguez B, Post AB, Asaad R, Heinzel FP, Valdez H, Tary-Lehmann M, Anthony DD.  Accessory cell dependent NK cell mediated PBMC IFN-gamma production is defective in HIV infection.  Clin Immunol. 2009. May;131(2):288-97.
  • Conry SJ, Milkovich KA, Yonkers NL, Rodriguez B, Bernstein HB, Asaad R, Heinzel FP, Tary-Lehmann M, Lederman MM, Anthony DD. Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function.  J Virol. 2009. Nov;83(21):11175-87.
  • Aung H., Sherman J., Tary-Lehmann M. and Toossi Z. Production of transforming growth factor-beta 1 (TGF-β1) expression in human monocytes infected with Mycrobacterium Avium at a single cell level by ELISPOT assay. J. Immunol. Methods. 2002. 259:25-32.
  • Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS. Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool. Am. J. Transplant. 2002. 2:857.
  • Schwander SK, Torres M, Carranza CC, Escobedo D, Tary-Lehmann M, Anderson P, Toossi Z, Ellner JJ, Rich EA, Sada E. Pulmonary mononuclear cell responses to antigens of Mycobacterium tuberculosis in healthy household contacts of patients with active tuberculosis and healthy controls from the community. J. Immunol. 2000. 165:1479.
  • Schwander, SK, Torres M, E. Sada, C. Carranza, E. Ramos, Tary-Lehmann M, R. S. Wallis, J. Sierra, and E. A. Rich. Enhanced responses to Mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis. J. Infect. Dis. 1998. 178:1434.
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Vaccine and Adjuvants

  • Tigno JT, Lehmann PV, Tary-Lehmann M. Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cellsJ. Immunol. 2009. 183(9): 5654-61.
  • Tigno JT, Lehmann PV, Tary-Lehmann M. Dissociated induction of cytotoxicity and DTH  by CFA and CpG. J. Immunother. 2009. 32:389
  • Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M. Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals. AIDS. 2005. 19:1967.
  • Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, Anthony DD. CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases. J. Immunol. Methods. 2004. 285:223.
  • Eisenberg JC, Czinn SJ, Garhart CA, Redline RW, Bartholomae WC, Gottwein JM, Nedrud JG, Emancipator SE, Boehm BO, Lehmann PV, Blanchard TG. Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice. Infect. Immun. 2003. 71:1820.
  • Lonsdorf AS, Kuekrek H, Stern BV, Boehm BO, Lehmann PV, Tary-Lehmann M. Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunityJ. Immunol. 2003. 171:3941.
  • Stern BV, Boehm BO, Tary-Lehmann M. Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity. J. Immunol. 2002. 168:6099.
  • Gottwein JM, Blanchard TG, Targoni OS, Eisenberg JC, Zagorski BM, Redline RW, Nedrud JG, Tary-Lehmann M, Lehmann PV, Czinn SJ. Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund’s adjuvant by systemic immunization. J. Infect. Dis. 2001. 184:308.
  • Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant. J. Immunol. 2000. 164:5771.
  • Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, H. Radeke, Heeger PS, Trezza RP, F. P. Heinzel, Forsthuber T, Lehmann PV. Adjuvant-guided type-1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response. J. Immunol. 1999. 162:3942.
  • Chu RS, Targoni OS, Krieg AM, Lehmann PV, Harding CV. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med. 1997. 186:1623.
  • Forsthuber T, Yip HC, Lehmann PV. Induction of TH1 and TH2 immunity in neonatal mice. Science. 1996. 271:1728. 
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Autoimmunity

  • Wunsch M, Hohmann C, Miles B, Rostermund C, Lehmann PV, Schroeter M, Bayas A, Ulzheimer J, Mäurer M, Ergün S, Kuerten S. The correlation between the virus- and brain antigen-specific B cell response in the blood of patients with Multiple Sclerosis. Viruses. 2016. 8, 105.
  • Kuerten S, Pommerschein G, Barth SK, Hohmann C, Miles B, Sammer FW, Duffy CE, Wunsch M, Rovituso DM, Schroeter M, Addicks K, Kaiser CC, Lehmann PV. Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood. Clin. Immunol. 2014. 152(1-2):20-24.
  • Kuerten S, Wunsch M, Lehmann PV. Longitudinal T cell-derived IFN-γ/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis.J. Immunol. Methods. 2013. 398-399:68-75.
  • Kuerten S, Addicks K, Lehmann PV. Studies on the CNS histopathology of EAE and its correlation with clinical and immunological parameters. In: Weissert R (Ed.), Experimental Autoimmune Encephalomyelitis – Models, Disease Biology and Experimental Therapy. Rijeka: Intech 2012.
  • Karulin AY, Quast S, Hesse M, Lehmann PV. Neuroantigen-specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages. Cells. 2012. 1:576-596.
  • Batoulis H, Uhl M, Addicks K, Lehmann PV, Kuerten S. The Magnitude of the Antigen-specific T Cell Response is Separated from the Severity of Spinal Cord Histopathology in Remitting-relapsing Experimental Autoimmune Encephalomyelitis. Glia. 2012. 60:794-805.
  • Zhang W, Tan J, Liu Y, Li W, Gao Q, Lehmann PV. T and B Cell Immunity is not Essential for the Induction of Proliferative Vitreoretinopathy. Eye. 2012. 26:872-881.
  • Kuerten S and Lehmann PV. The Immune Pathogenesis of Experimental Autoimmune Encephalomyelitis – Lessons Learned for Multiple Sclerosis? Journal of Interferon & Cytokine Research. 2011. 31:907-16.
  • Kuerten S, Gruppe TL, Laurentius LM, Kirch C, Tary-Lehmann M, Lehmann PV, Addicks K. Differential patterns of spinal cord pathology induced by MP4, MOG peptide 35-55, and PLP peptide 178-191 in C57BL/6 mice. APMIS. 2011. Jun;119(6):336-46.
  • Kuerten S, Pauly R, Rottlaender A, Rodi M, Gruppe TL, Addicks K, Tary-Lehmann M, and Lehmann PV.Myelin-reactive antibodies mediate the pathology of MBP-PLP fusion protein MP4-induced EAE. Clin. Immunol. 2011. 140: 54-62.
  • Javeri S, Rodi M, Tary-Lehmann M, Lehmann PV, Addicks K, Kuerten S. Involvement of brain-derived neurotrophic factor (BDNF) in MP4-induced autoimmune encephalomyelitis. Clin Immunol. 2010. 137(2):181-9.
  • Kuerten S, Rottlaender A, Rodi M, Velasco VB Jr, Schroeter M, Kaiser C, Addicks K, Tary-Lehmann M, Lehmann PV. The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood. Clin. Immunol. 2010. 137(3):422-32.
  • Kuerten S, Rodi M, Javeri S, Gruppe TL, Tary-Lehmann M, Lehmann PV, Addicks K. Delineating the impact of neuroantigen vs genetic diversity on MP4-induced EAE of C57BL/6 and B6.129 mice.APMIS. 2009; 117(12):923-35. 
  • Tigno JT, Lehmann PV, Tary-Lehmann M. Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cells.J. Immunol. 2009. 183(9): 5654-61.
  • Hattier T and Lehmann PV. Making the jump to high-throughput microbial detection. Medlab. 2008. 3, 30-32.
  • Kuerten S, Javeri S, Tary-Lehmann M, Lehmann PV, and Angelov DN. Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis. Clin. Immunol. 2008. 129: 256-267.
  • Hofstetter HH, Kovalovski A, Shive C, Lehmann PV, Forsthuber TG. Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood. J. Neuroimmunol. 2007. 187: 20-30.
  • Kuerten S, Kostova-Bales DA, Frenzel LP, Tigno JT, Tary-Lehmann M, Doychin N. Angelov, Lehmann PV.MP4- and MOG:35-55-induced EAE in C57BL/6 mice differentially targets brain, spinal cord and cerebellum. J. Neuroimmunol. 2007. 189:31-40.
  • Hofstetter HH, Toyka KV, Tary-Lehmann M, Lehmann PV. Kinetics and Organ Distribution of IL-17-Producing CD4 Cells in Proteolipid Protein 139-151 Peptide-Induced Experimental Autoimmune Encephalomyelitis of SJL Mice. J. Immunol. 2007. 178:1372.
  • Lichtenegger FS, Kuerten S, Faas S, Boehm BO, Tary-Lehmann M, Lehmann PV. Dissociation of EAE protective effect and allergic side reactions in tolerization with neuroantigen. J. Immunol. 2006. 178:4749-56.
  • Kuerten S, Lichtenegger FS, Faas S, Angelov DN, Tary-Lehmann M, Lehmann PV. MBP-PLP fusion protein-induced EAE in C57BL/6 mice. J. Neuroimmunol. 2006. 177(1-2):99-111.
  • Hofstetter HH, Targoni OS, Karulin AY, Forsthuber TG, Tary-Lehmann M, Lehmann PV. Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis? J. Immunol. 2005. 174:4598-605.
  • Hofstetter HH, Karulin AY, Forsthuber TG, Ott PA, Tary-Lehmann M, Lehmann PV. The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice. J. Neuroimmunol. 2005. 170:105.
  • Ott PA, Anderson MR, Tary-Lehmann M, Lehmann PV. CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes. Cell Immunol. 2005. 235:1. 
  • Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV. Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin. Immunol. 2005. 115:102. 
  • Darabi K, Karulin AY, Boehm BO, Hofstetter HH, Fabry Z, LaManna JC, Chavez JC, Tary-Lehmann M, Lehmann PV. The third signal in T cell-mediated autoimmune disease? J. Immunol. 2004. 173:92-99.
  • Herzog BA, Ott PA, Dittrich MT, Quast S, Karulin AY, Kalbacher H, Karges W, Tary-Lehmann M, Lehmann PV, Boehm BO, Durinovic-Bello I. Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects. J. Autoimmun. 2004. 23:45.
  • Ott PA, Dittrich MT, Herzog BA, Guerkov R, Gottlieb PA, Putnam AL, Durinovic-Bello I, Boehm BO, Tary-Lehmann M, Lehmann PV. T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading. J. Clin. Immunol. 2004. 24:327.
  • Hofstetter HH, Sewell DL, Liu F, Sandor M, Forsthuber T, Lehmann PV, Fabry Z. Autoreactive T cells promote post-traumatic healing in the central nervous system. J. Neuroimmunol. 2003. 134:25.
  • Meierhoff G, Ott PA, Lehmann PV, Schloot NC. Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes. Diabetes Metab. Res. Rev. 2002. 18:367.
  • Radeke HH, Tschernig T, Karulin A, Schumm G, Emancipator SN, Resch K, Tary-Lehmann M. CD4+ T cells recognizing specific antigen deposited in glomeruli cause glomerulonephritis-like kidney injury. Clin. Immunol. 2002. 104:161.
  • Targoni OS, Baus J, Hofstetter HH, Hesse MD, Karulin AY, Boehm BO, Forsthuber TG, Lehmann PV.Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis. J. Immunol. 2001. 166:4757.
  • Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant. J. Immunol. 2000. 164:5771.
  • Lehmann PV. Selective peptidomimetic blockers of autoantigen presentation: a novel therapeutic approach to autoimmune disease. Trends Pharmacol. Sci. 2000. 21:79.
  • Pelfrey CM, Rudick RA, Cotleur AC, Lee JC, Tary-Lehmann M, Lehmann PV. Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production. J. Immunol. 2000. 165:1641.
  • Targoni OS, Tary-Lehmann M, Lehmann PV. Prevention of murine EAE by oral hydrolytic enzyme treatment.J. Autoimmun. 1999. 12:191.
  • Birnbaum G, Kotilinek L, Miller SD, Raine CS, Gao YL, Lehmann PV, Gupta RS. Heat shock proteins and experimental autoimmune encephalomyelitis. II: environmental infection and extra-neuraxial inflammation alter the course of chronic relapsing encephalomyelitis. J. Neuroimmunol. 1998. 90:149.
  • Gross DM, Forsthuber T, Tary-Lehmann M, Etling C, Ito K, Nagy ZA, Field JA, Steere AC, Huber BT.Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis.Science. 1998. 281:703.
  • Lehmann PV, Targoni OS, Forsthuber TG. Shifting T-cell activation thresholds in autoimmunity and determinant spreading. Immunol. Rev. 1998. 164:53.
  • Lehmann PV. The fate of T cells in the brain: veni, vidi, vici and veni, mori. Am. J. Pathol. 1998. 153:677.
  • Targoni OS, Lehmann PV. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 1998. 187:2055.
  • Tian J, Lehmann PV, D. L. Kaufman. Determinant spreading of T helper cell 2 (Th2) responses to pancreatic islet autoantigens. J. Exp. Med. 1997. 186:2039. 
  • Tian J, Atkinson MA, Clare-Salzler M, Herschenfeld A, Forsthuber T, Lehmann PV, Kaufman DL. Nasal administration of glutamate decarboxylase (GAD65) peptides induces Th2 responses and prevents murine insulin-dependent diabetes. J. Exp. Med. 1996. 183:1561. 
  • Tian J, Clare-Salzler M, Herschenfeld A, Middleton B, Newman D, Mueller R, Arita S, Evans C, Atkinson MA, Mullen Y, Sarvetnick N, Tobin AJ, Lehmann PV, Kaufman DL. Modulating autoimmune responses to GAD inhibits disease progression and prolongs islet graft survival in diabetes-prone mice. Nat. Med. 1996. 2:1348.
  • Tian J, Lehmann PV, Kaufman DL. T cell cross-reactivity between coxsackievirus and glutamate decarboxylase is associated with a murine diabetes susceptibility allele. J. Exp. Med. 1994. 180:1979.
  • Kaufman DL, Clare-Salzler M, Tian J, Forsthuber T, Ting GS, Robinson P, Atkinson MA, Sercarz EE, Tobin AJ, Lehmann PV. Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes. Nature. 1993. 366:69.
  • Lehmann PV, Sercarz EE, Forsthuber T, Dayan CM, Gammon G. Determinant spreading and the dynamics of the autoimmune T-cell repertoire. Immunol. Today. 1993. 14:203.
  • Sercarz EE, Lehmann PV, Ametani A, Benichou G, Miller A, Moudgil K. Dominance and crypticity of T cell antigenic determinants. Annu. Rev. Immunol. 1993. 11:729.
  • Lehmann PV, Forsthuber T, Miller A, Sercarz EE. Spreading of T-cell autoimmunity to cryptic determinants of an autoantigen. Nature. 1992. 358:155.
  • Falcioni F, Dembic Z, Mulller S, Lehmann PV, Nagy ZA. Flexibility of the T cell repertoire. Self tolerance causes a shift of T cell receptor gene usage in response to insulin. J. Exp. Med. 1990. 171:1665.
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Tumor Immunology

  • Lehmann PV, Sundararaman S. Chapter: When results of T cell immune monitoring match / do not match clinical outcomes of tumor vaccine trials – what more could and should we measure? In: The Tumor ImmunoEnvironment, ed. MR Shurin, V Umansky & AM Malyguine, 2013. Chapter 32, 725-740, Springer.
  • Butterfield LH, Palucka AK, Britten CM, Dhodapkar MV, Hakansson L, Janetzki S, Kawakami Y, Kleen TO, Lee PP, Maccalli C, Maecker HT, Maino VC, Maio M, Malyguine A, Masucci G, Pawelec G, Potter DM, Rivoltini L, Salazar LG, Schenedel DJ, Slingluff CL, Song W, Stroncek DF, Tahara H, Thurin M, Trinchieri G, van Der Burg SH, Whiteside TL, Wigginton JM, Marincola F, Khleif S, Fox BA, Disis ML.Recommendations from the iSBTc-SITC/FDA/NCI Workshop on Immunotherapy BioMarkers. Clin Cancer Res. 2011. 17:3064-3076.
  • Schlingmann TR, Rininsland FH, Bartholomae W, Boehm BO, Lehmann PV, Tary-Lehmann M.Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself. Clin. Immunol. 2009. 133(1): 45-51.
  • Bartholomae WC., Rininsland FH, Eisenberg JC, Boehm BO, Lehmann PV, Tary-Lehmann M. T cell immunity induced by live, necrotic, and apoptotic tumor cells. J. Immunol. 2004. 173:1012.
  • Lonsdorf AS, Kuekrek H, Stern BV, Boehm BO, Lehmann PV, Tary-Lehmann M. Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunity. J. Immunol. 2003. 171:3941.
  • Stern BV, Boehm BO, Tary-Lehmann M. Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity. J. Immunol.  2002. 168:6099.
  • Uittenbogaart CH, Anisman DJ, Tary-Lehmann M, Vollger LW, Breit TM, Van Dongen JJ, Saxon A. The SCID mouse environment causes immunophenotypic changes in human immature T-cell lines.Int. J. Cancer.  1994. 56:546. 
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Transplantation Immunology

  • Fanning LR, Hegerfeldt Y, Tary-Lehmann M, Lesniewski M, Maciejewski J, Weitzel RP, Kozik M, Finney M, Lazarus HM, Paul P, Ratajczak MZ, Meyerson HJ, Laughlin MJ. Allogeneic transplantation of multiple umbilical cord blood units in adults: role of pretransplant-mixed lymphocyte reaction to predict host-vs-graft rejection. Leukemia. 2008 Sep;22(9):1786-90.
  • Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice.BMC. Musculoskelet. Disord. 2006. 7:32
  • Hetherington VJ, Kawalec JS, Dockery DS, Targoni OS, Lehmann PV, Nadler D. Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors. BMC. Musculoskelet. Disord. 2005. 6:36.
  • Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ. Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation. Transplantation. 2005. 80:1316. 
  • Hricik DE, Rodriguez V, Riley J, Bryan K, Tary-Lehmann M, Greenspan N, Dejelo C, Schulak JA, Heeger PS. Enzyme linked immunosorbent spot (ELISPOT) assay for interferon-gamma independently predicts renal function in kidney transplant recipients. Am. J. Transplant. 2003. 3:878.
  • Kadereit S, Junge GR, Kleen T, Kozik MM, Kaminski BA, um-Woods K, Fu P, Tary-Lehmann M, Laughlin MJ. Deficient IFN-gamma expression in umbilical cord blood (UCB) T cells can be rescued by IFN-gamma-mediated increase in NFATc2 expression.J. Clin. Immunol. 2003. 23:485. 
  • Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS. Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool. Am. J. Transplant. 2002. 2:857.
  • Baron ED, Heeger PS, Hricik DE, Schulak JA, Tary-Lehmann M, Stevens SR. Immunomodulatory effect of extracorporeal photopheresis after successful treatment of resistant renal allograft rejection. Photodermatol. Photoimmunol. Photomed. 2001. 17:79.
  • Heeger PS, Valujskikh A, Lehmann PV. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen. J. Immunol. 2000. 165:1278.
  • Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant. J. Immunol. 2000. 164:5771.
  • Heeger PS, Greenspan NS, Kuhlenschmidt S, Dejelo C, Hricik DE, Schulak JA, Tary-Lehmann M.Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes. J. Immunol. 1999. 163:2267.
  • Matesic D, Lehmann PV, Heeger PS. High-resolution characterization of cytokine-producing alloreactivity in naive and allograft-primed mice. Transplantation. 1998. 65:906.
  • Tary-Lehmann, M., Hricik DE, Justice AC, Potter NS, Heeger PS. Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure. Transplantation. 1998. 66:219.
  • Lehmann PV, Matesic D, Benichou G, Heeger PS. Induction of T helper 2 immunity to an immunodominant allopeptide. Transplantation. 1997. 64:292.
  • Lehmann PV. Immunomodulation by proteolytic enzymes. Nephrol. Dial. Transplant. 1996. 11:952.
  • Tary-Lehmann M., Saxon A, Lehmann PV. The human immune system in hu-PBL-SCID mice. Immunol. Today. 1995. 16:529.
  • Benichou G., Fedoseyeva E, Lehmann PV, Olson CA, Geysen HM, McMillan M, Sercarz EE. Limited T cell response to donor MHC peptides during allograft rejection. Implications for selective immune therapy in transplantation. J. Immunol. 1994. 153:938. 
  • Tary-Lehmann M, Lehmann PV, Schols D, Roncarolo MG, Saxon A. Anti-SCID mouse reactivity shapes the human CD4+ T cell repertoire in hu-PBL-SCID chimeras. J. Exp. Med. 1994. 180:1817. 
  • Tary-Lehmann M., A. Saxon. Human mature T cells that are anergic in vivo prevail in SCID mice reconstituted with human peripheral blood. J. Exp. Med. 1992. 175:503.
  • Lehmann PV, K. Drexler, Tary-Lehmann M, F. Falcioni, U. Hurtenbach, and Nagy ZA. Graft-versus-host resistance induced by class II major histocompatibility complex-specific T cell clones. J. Exp. Med. 1991. 173:333.
  • Saxon, A., E. Macy, K. Denis, Tary-Lehmann M, O. Witte, and J. Braun. Limited B cell repertoire in severe combined immunodeficient mice engrafted with peripheral blood mononuclear cells derived from immunodeficient or normal humans. J. Clin. Invest. 1991. 87:658.
  • Lehmann PV, G. Schumm, D. Moon, U. Hurtenbach, F. Falcioni, Mulller S, and Nagy ZA. Acute lethal graft-versus-host reaction induced by major histocompatibility complex class II-reactive T helper cell clones. J. Exp. Med. 1990. 171:1485.
  • Tary-Lehmann, M., A. G. Rolink, Lehmann PV, Nagy ZA, and U. Hurtenbach. Induction of graft versus host-associated immunodeficiency by CD4+ T cell clones. J. Immunol. 1990. 145:2092.
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Infectious and Other Diseases

  • Moldovan I, Targoni OS, Zhang W, Sundararaman S, Lehmann PV. How frequently are predicted peptides actually recognized by CD8 cells?Cancer Immunology, Immunotherapy. 2016. DOI 10.1007/s00262-016-1840-7.
  • Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV. Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity. Cells. 2015; 7:4414-4437.
  • Chenggang J, Roen DR, Lehmann PV, Kellermann GH. An enhanced ELISPOT assay for sensitive detection of antigen-specific T cell responses to Borrelia burgdorferi. Cells, 2013, 607-620.
  • Nowacki T, Bettenworth D, Ross M, Heidemann J, Lehmann PV, Lügering A. Cytomegalovirus (CMV)-specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease. Cells. 2012. 1:35-50.
  • Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV. How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides? Viruses. 2012. 4:2636-2649.
  • Osorio JE, Brewoo JN, Silengo SJ, Arguello J, Moldovan IR, Tary-Lehmann M , Powell TD, Livengood JA, Kinney RM, Huang CYH and Stinchcomb DT. Efficacy of a Tetravalent Chimeric Dengue Vaccine (DENVax) in Cynomolgus Macaques. Am. J. Trop. Med. Hyg. 84(6), 2011. pp. 978–987.
  • Ramaswami B, Popescu I, Macedo C, Luo C, Shapiro R, Metes D, Chalasani G, and Randhawa PS.The Polyomavirus BK large T-antigen-derived peptide elicits an HLA-DR promiscuous and polyfunctional CD4+ T cell response. Clin. Vaccine Immunol. 2011. 18(5):815-824.
  • Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M. Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer’s disease. Neurobiol Aging. 2010. 31(10):1732-42.
  • Yonkers NL, Milkovich KA, Rodriguez B, Post AB, Asaad R, Heinzel FP, Valdez H, Tary-Lehmann M, Anthony DD.  Accessory cell dependent NK cell mediated PBMC IFN-gamma production is defective in HIV infection.  Clin Immunol. 2009. May;131(2):288-97.
  • Conry SJ, Milkovich KA, Yonkers NL, Rodriguez B, Bernstein HB, Asaad R, Heinzel FP, Tary-Lehmann M, Lederman MM, Anthony DD. Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function. J Virol. 2009. Nov;83(21):11175-87.
  • Mirano-Bascos D,  Tary-Lehmann M and Landry SJ. Antigen structure influences helper T-cell epitope dominance in the human immune response to HIV envelope glycoprotein gp120. Eur J Immunol. 2008. May;38(5):1231-7.
  • Kuerten S, Schlingmann T, Rajasalu T, Angelov DN, Lehmann PV, Tary-Lehmann M. Lack of Disease Specificity Limits the Usefulness of in vitro Costimulation in HIV- and HCV-Infected Patients. Clin. Dev. Immunol. 2008. 590941.
  • Kuerten S, Asaad RJ, Schoenberger SP, Lehmann PV, Tary-Lehmann M. The TRAIL of helpless CD8+ T cells in HIV infection. AIDS Res Hum Retroviruses. 2008. 24: 1-9.
  • Kuerten S, Kleen T, Assad RJ, Lehmann PV, Tary-Lehmann M. Dissociated production of perforin, granzyme B and IFN-γ by HIV-specific CD8+ cells in HIV infection.  AIDS Research and Human Retroviruses. 2007. 24: 62-71.
  • Yonkers NL, Rodriguez B, Post AB, Asaad R, Jones L, Lederman MM, Lehmann PV, Anthony DD. HIV Coinfection Impairs CD28-Mediated Costimulation of Hepatitis C Virus-Specific CD8 Cells. J. Infect. Dis. 2006. 194:391.
  • Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D'Souza MP, and the ELISPOT Collaborative Study Group. Results of an ELISPOT proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials. AIDS Research & Human Retroviruses. 2005. 21: 68-81.
  • Berner BR, Tary-Lehmann M, Yonkers NL, Askari AD, Lehmann PV, Anthony DD. Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE. Cell Immunol. 2005. 235:29.
  • Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M. Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals. AIDS. 2005. 19:1967.
  • Anthony DD, Yonkers NL, Post AB, Asaad R, Heinzel FP, Lederman MM, Lehmann PV, Valdez H.Selective impairments in dendritic cell-associated function distinguish hepatitis C virus and HIV infection. J. Immunol. 2004. 172:4907.
  • Kleen TO, Asaad R, Landry SJ, Boehm BO, Tary-Lehmann M. Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection. AIDS. 2004. 18:383.
  • Anthony DD, Lehmann PV. T-cell epitope mapping using the ELISPOT approach. Methods. 2003. 29:260.
  • Eisenberg JC, Czinn SJ, Garhart CA, Redline RW, Bartholomae WC, Gottwein JM, Nedrud JG, Emancipator SE, Boehm BO, Lehmann PV, Blanchard TG. Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice. Infect. Immun. 2003. 71:1820.
  • Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.J. Immunol. Methods. 2003. 279:111. 
  • Li R, Liu T, Yoshihiro F, Tary-Lehmann M, Obrenovich M, Kuekrek H, Kang SC, Pan T, Wong BS, Medof ME, Sy MS. On the same cell type GPI-anchored normal cellular prion and DAF protein exhibit different biological properties. Biochem. Biophys. Res. Commun. 2003. 303:446.
  • Aung H, Sherman J, Tary-Lehmann M, Toossi Z. Production of transforming growth factor-beta 1 (TGF-β1) expression in human monocytes infected with Mycrobacterium Avium at a single-cell level by ELISPOT assay. J. Immunol. Methods. 2002. 259:25-32.
  • Anthony DD, Valdez H, Post AB, Carlson NL, Heeger PS, Lehmann PV. Comprehensive determinant mapping of the hepatitis C-specific CD8 cell repertoire reveals unpredicted immune hierarchy. Clin. Immunol. 2002. 103:264.
  • Valdez, H., Carlson NL, Post AB, Asaad R, Heeger PS, Lederman MM, Lehmann PV, Anthony DD. HIV long-term non-progressors maintain brisk CD8 T cell responses to other viral antigens. AIDS. 2002. 16:1113.
  • Anderson MR, Tary-Lehmann M. Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell-dependent, but disease susceptibility is defined by the non-T-cell compartment. Clin. Immunol. 2001. 98:85.
  • Gottwein JM, Blanchard TG, Targoni OS, Eisenberg JC, Zagorski BM, Redline RW, Nedrud JG, Tary-Lehmann M, Lehmann PV, Czinn SJ. Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund’s adjuvant by systemic immunization. J. Infect. Dis. 2001. 184:308.
  • Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol. 2000. 164:3723.
  • Rininsland FH, Helms T, Asaad RJ, Boehm BO, Tary-Lehmann M. Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J. Immunol. Methods. 2000. 240:143.
  • Schwander SK, Torres M, Carranza CC, Escobedo D, Tary-Lehmann M, Anderson P, Toossi Z, Ellner JJ, Rich EA, Sada E. Pulmonary mononuclear cell responses to antigens of Mycobacterium tuberculosis in healthy household contacts of patients with active tuberculosis and healthy controls from the community. J. Immunol. 2000. 165:1479.
  • Gross DM, Forsthuber T, Tary-Lehmann M, Etling C, Ito K, Nagy ZA, Field JA, Steere AC, Huber BT.Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis. Science. 1998. 281:703.
  • Schwander SK, Torres M, Sada E, Carranza C, Ramos E, Tary-Lehmann M, Wallis RS, Sierra J, Rich EA.Enhanced responses to Mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis. J. Infect. Dis. 1998. 178:1434.
  • Le Grice SF, Whalen CC, Hom DL, Lehmann PV, Walker CJ. Perspective: research highlights at the Case Western Reserve University Center for AIDS Research. Learning from the past and looking toward the future. AIDS Res. Hum. Retroviruses.  1996. 12:1659. 
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ELISPOT-related Reviews/Book Chapters

  • Lehmann PV, Sundararaman S. Chapter: When results of T cell immune monitoring match or do not match clinical outcomes of tumor vaccine trials: What more could and should we measure? The Tumor Immunoenvironment. Springer. 2013 725-740. Editors, Shurin M, Umansky V, and Malyguine AM.
  • Lehmann PV, Zhang W. Chapter: Unique Strengths of ELISPOT for T Cell Diagnostics. Methods and Protocols, Handbook of ELISPOT. Humana Press, 2nd Edition. Editor, Alexander E. Kalyuzhny. Methods in Molecular Biology. 2011. 792: 3-23.
  • Karulin AY, Lehmann PV. Chapter: How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity. Methods and Protocols, Handbook of ELISPOT. Humana Press, 2nd Edition. Editor, Alexander E. Kalyuzhny. Methods in Molecular Biology.2011. 792: 125-143.
  • Zhang W, Lehmann PV. Chapter: Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles. Methods and Protocols, Handbook of ELISPOT. Humana Press, 2nd Edition. Editor, Alexander E. Kalyuzhny. Methods in Molecular Biology. 2011. 792: 155-171.
  • Dittrich M, Lehmann PV. Chapter: Statistical Analysis of ELISPOT Assays. Methods and Protocols, Handbook of ELISPOT. Humana Press, 2nd Edition. Editor, Alexander E. Kalyuzhny. Methods in Molecular Biology. 2011. 792: 173-183.
  • Tary-Lehmann M, Hamm CD, Lehmann PV. Chapter: Validating reference samples for comparison in a regulated ELISPOT assay. Validation of Cell-based Assays in the GLP Setting. Editors, Uma Prabhakar and Marian Kelley. 2008. ISBN: 97804700287662008. 
  • Lehmann PV. Chapter: Image Analysis and Data Management of ELISPOT Assay Results.Methods and Protocols, Handbook of ELISPOT. Humana Press, 2005. Methods in Molecular Biology. 2005. 302: 117-132 
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