The SpotStat® software suite was developed for the statistical analysis of ELISPOT/FLUOROSPOT data in immune monitoring, vaccine testing, and other fields of immunological and medical research.

Our SpotStat® software suite implements a number of powerful parametric and non-parametric statistical methods for easy, streamlined analysis of data generated by the CTL ImmunoSpot® platform. The primary function of SpotStat® is to provide statistically-validated discrimination between antigen-induced responses and negative controls. SpotStat® was specifically designed to perform multiple comparison tests using a number of family-wise error correction methods, when multiple positive responses (e.g. peptide screening in T cell assays) are compared to a single negative control for the same subject (donor). SpotStat® also offers a set of “descriptive statistics” methods including three tests for data normality. These tests are performed on each entered set of data and allows users to choose between parametric or non-parametric methods for final data interpretation and decision-making. Results of all statistical tests are exported into Excel®, facilitating further data analysis and graphical outputs.


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